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Pan, Sitansu
- Characterization of Antagonistic Potential of Trichoderma Spp. against some Soil Borne Plant Pathogens
Abstract Views :259 |
PDF Views:116
Authors
Affiliations
1 Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal - 741252, IN
2 Division of Field Crops, Central Agricultural Research Institute, Port Blair, Andaman-744101, IN
1 Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal - 741252, IN
2 Division of Field Crops, Central Agricultural Research Institute, Port Blair, Andaman-744101, IN
Source
Journal of Biological Control, Vol 22, No 1 (2008), Pagination: 43-49Abstract
Ten isolates of Trichoderma spp. isolated from rhizosphere of different crops were evaluated for their antagonistic potential against five soil borne plant pathogens, viz., Rliizoctonia solani, Fusarium oxysporum f sp radicis-lycopersici, Macrophomina phaseolina and Sclerotium rolfsii using dual culture technique and production of volatile and non-volatile antibiotics. Sclerotial antagonism by the biocontrol strains was tested with R. solani and S. rolfsii. The isolate T2, T4 and T7 against Pythium sp., T1, T7 and T10 against F. oxysporum f. sp radicislycopersici, T3 and T9 against R. solani, T7 against S. rolfsii and T6 against M. phaseolina were the most efficient. The highest percentage inhibition of respective pathogen through the production of certain metabolites of Trichoderma isolates was recorded with T7 against Pythium sp, and Fusarium oxysporum sp. radicis-lycopersici, T1 against R. solani, T5 and T6 against S. rolfsii and T9 isolate against M. phaseolina. Trichoderma isolate T9 and T2 were most effective in inhibition of sclerotia formation, production and germination of sclerotia of R. solani and S. rolfsii, respectively.Keywords
Antagonistic Potential, Sclerotial Antagonism, Trichoderma Spp., Volatile And Nonvolatile Metabolites.- Variability in Production of Extracellular Hydrolytic Enzymes by Trichoderma spp. and Induction of Resistance in Gram (cicer arietinum)
Abstract Views :227 |
PDF Views:112
Authors
Affiliations
1 Division of Field Crops, Central Agricultural Research Institute, Port Blair- 744101, Andaman & Nicobar Islands, IN
2 Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia - 741252, West Bengal, IN
1 Division of Field Crops, Central Agricultural Research Institute, Port Blair- 744101, Andaman & Nicobar Islands, IN
2 Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia - 741252, West Bengal, IN
Source
Journal of Biological Control, Vol 22, No 1 (2008), Pagination: 57-66Abstract
Ten isolates of Trichoderma spp. isolated from rhizosphere region Of different crops were evaluated for their ability to produce extracellular hydrolytic enzymes, viz., β-1, 3 glucanase, chitinase and cellulase enzymes with different concentrations ef carbon sources, pM, and temperature levels and the induction of systemic resistance in gram seedlings. Highest chitinase and β-1, 3 glucanase activity were observed in TR2 isolate whereas TH2 isolate exhibited highest activity of cellulase. TH1 and TH5 were the next best isolates in their chtinase enzyme activity. Similarly, TR1 and TH1 isolates followed the TR2 for β-1, 3-glucanase activity. The activity of cellulase was highest in TH2 isolate followed by TH5 and TR2, which were statistically significant. The enzyme β-1,3 glucanase was produce in media with pH 4.0 to 8.0 with an optimum pH5.5. The optimum pH for chitinase and cellulase enzyme activity was recorded at 5.0. With the variable temperatures, i.e., 20, 25, 30, 35 and 40°C, the enzyme activities of all three enzymes showed highest at 30°C. The activities of enzymes β-1,3 glucanase and chitinase was recorded highest at 3.0 and 4.0% concentrations of glucan and chitin, respectively whereat 0.75% concentration of cellulose showed highest cellulase activity. The highest induced resistance by Trichoderma isolates in gram seedlings, was recorded by TR1, through the increase activity of PAL and β-1,3 glucanase and TH1 by enhance activity of peroxidase enzyme.Keywords
β,-1,3 Glucanase, Cellulase, Chi Tinase, Induced ResistanceTrichodermaspp.- Biological Management of Root and Collar Rot of Cauliflower (Rhizoctonia solani) by a Talc-Based formulation of Trichoderma harzianum Rifai
Abstract Views :166 |
PDF Views:132
Authors
Affiliations
1 Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, 741252, West Bengal, IN
1 Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, 741252, West Bengal, IN
Source
Journal of Biological Control, Vol 22, No 2 (2008), Pagination: 483-486Abstract
The study on the management of collar rot of cauliflower, caused by Rhizoctoniu solani by a talc-based formulation of Trichoderma harzianum, was carried out during the early rabi seasons of 2005-06 and 2006-07 under field condition at Mohanpur, West Bengal with isolates of T. harzinum. The seedling dip and soil application of T. harzianum (talc-based) @ 200g per plot significantly reduced the disease incidence (63.6%) at 50 DAT followed by pre-transplanting seedling tip in T. harzianum spore suspension @ 5g/l combined with soil application of talc formulation @ 100g/plot (52.7%) and soil application of the talc formulation of T. Iiarzianum at 200g/plot (50.9%) treatment and there was corresponding yield increase in the respective treatments. The highest population density of T. harzianum was recorded in the plot treated with seedling tip and soil application (210 cfu/g soil) and the lowest in the control plot (7.5 cfu/g soil).Keywords
Biological Management, Cauliflower, Root and Collar Rot, Talc-Based Formulation T. harzianum.- Factors Affecting Colonisation of Groundnut Stem by Gliocladium virens Miller, Giddens and Foster in Soil
Abstract Views :206 |
PDF Views:156
Authors
D. K. Saha
1,
Sitansu Pan
1
Affiliations
1 Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya Mohanpur, Nadia 741 252, West Bengal, IN
1 Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya Mohanpur, Nadia 741 252, West Bengal, IN
Source
Journal of Biological Control, Vol 10, No 1&2 (1996), Pagination: 103-109Abstract
Ability of Gliocladium virens Miller, Giddens and Foster to colonise groundnut stem pieces used as organic bait in soil was studied under varying conditions like inoculum density, soil pH, soil moisture and temperature. High temperature (40°C) was lethal for the saprophytic activities of the antagonist as colonisation of the bait was completely inhibited. In air dry soil (ca. 6.0% mhc) colonisation of bait was low (ca. 5.0 - 8.0 %) particularly at lower temperature (20°C) and low level of inoculum (20 mg/100 g soil). Acid soil (pH 5.6) at 50% soil moisture and 30°C temperature gave maximum colonisation of bait. Per cent colonisation of bait was less at either side of this temperature moisture optima.Keywords
Antagonist, Competitive Colonisation, Gliocladium virens, Inoculum, Organic Bait.- Effect of Soil pH and Soil Type on the Germinability of Phialospores of Gliocladium virens Millers, Giddens and Foster
Abstract Views :233 |
PDF Views:109
Authors
D. K. Saha
1,
Sitansu Pan
1
Affiliations
1 Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal 741252, IN
1 Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal 741252, IN
Source
Journal of Biological Control, Vol 9, No 1 (1995), Pagination: 37-40Abstract
Phialospores of four isolates of Gliocladium virens viz., 15GV1, 26GV1, 1AGV2 and IAGV6 responded differentially to soil fungistasis in different soils of West Bengal according to the properties of soils explored. The pH of soil appeared to be the cardinal factor. The effect of soil fungistasis was more pronounced in alkaline soil followed by neutral soil but gradually decreased due to reduction of soil pH to acidic level. Fungistasis was more in unsterilized soil compared to sterilised one.Keywords
Gliocladium virens, Phialospore, Fungistasis, Soil pH, Soil Type.- Development of an Encapsulated Prill Formulation of Gliocladium virens Miller, Giddens & Foster
Abstract Views :184 |
PDF Views:134
Authors
D. K. Saha
1,
Sitansu Pan
1
Affiliations
1 Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, Pin-741 252, IN
1 Department of Plant Pathology, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal, Pin-741 252, IN